{"id":3852,"date":"2022-11-05T13:47:42","date_gmt":"2022-11-05T13:47:42","guid":{"rendered":"https:\/\/wapcpharm.org\/journal\/?p=3852"},"modified":"2023-07-11T13:54:49","modified_gmt":"2023-07-11T13:54:49","slug":"west-african-journal-of-pharmacy-2022-33-1-72-79","status":"publish","type":"post","link":"https:\/\/wapcpharm.org\/journal\/west-african-journal-of-pharmacy-2022-33-1-72-79\/","title":{"rendered":"West African Journal of Pharmacy (2022) 33 (1) 72 – 79"},"content":{"rendered":"\t\t
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A new RP-HPLC method for metformin determination in human serum<\/b><\/p>

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Nasir Ibrahim , Amina J. Yusuf , Abdullahi M. Ismail , Musa Aminu , Awwalu Salisu<\/p>

1Department of Pharmaceutical and Medicinal Chemistry, Faculty of Pharmaceutical Sciences,\u00a0Usmanu Danfodiyo University, Sokoto, Nigeria.<\/span><\/p>

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2Department of Pharmaceutical and Medicinal Chemistry, Faculty of Pharmaceutical Sciences,\u00a0Ahmadu Bello University, Zaria, Nigeria.<\/span><\/p>

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Corresponding author: Nasir Ibrahim<\/p>

Email: thenasir25@gmail.com, nasir.ibrahim@udusok.edu.ng;<\/p>

Phone: +2348035169018<\/p>\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t

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ABSTRACT<\/h2>\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t
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Background:<\/strong> Anticoagulant in the sample bottles could form complex with metformin thereby affecting its concentration. This necessitates the need to develop HPLC method for determination of metformin in serum<\/p>

Objectives:<\/strong> This study was aimed at developing a simple, less tedious RP-HPLC method for the determination of metformin in human serum.<\/p>

Methods:<\/strong> Blank serum (2 mL) was spiked into solution containing 2 mL metformin (2-65 \u03bcg mL-1) and 1 mL caffeine (5.0 \u03bcg mL-1) as internal standard (IS) centrifuged at 3000 rpm for 5 minutes. Portion (0.5 mL) of the resultant solution was injected into the HPLC auto sample machine (Agilent 1260 infinity). The optimized conditions included a mobile phase of methanol-water (80:20 v\/v) containing 0.1 % orthophosphoric acid, isocratic elusion mode, an injection volume of 10 \u03bcL, flow rate of 1.2 mL min-1, at 35 \u00b0C and detection wavelength of 255 nm. Calibration curve (0.40 to 12.2 \u03bcg mL-1) was constructed by plotting the peak area ratios against their corresponding concentrations. The method was validated according to ICH guidelines.<\/p>

Results:<\/strong> Metformin and caffeine eluted at 1.29 and 1.42 minutes respectively. The method was precise (4.77 % RSD), accurate (% Er of 0.22 and % recovery of 99.78 %) with a linear calibration curve (r = 0.988). LOD and LOQ of the developed method are 1.93 and 5.87 \u03bcg\/mL respectively. All the parameters were within the acceptable limits.<\/p>

Conclusion:<\/strong> The developed method was found to be simple, precise, accurate and rapid for the routine therapeutic metformin monitoring in human serum.<\/p>

Keywords:<\/strong> Therapeutic metformin monitoring, RP-HPLC, serum, isocratic elution.<\/p>\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t

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\n\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\tDownload <\/span>\n\t\t<\/span>\n\t\t\t\t\t<\/a>\n\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t\t\t\t<\/div>\n\t\t","protected":false},"excerpt":{"rendered":"

A new RP-HPLC method for metformin determination in human serum \u00a0 Nasir Ibrahim , Amina J. Yusuf , Abdullahi M. Ismail , Musa Aminu , Awwalu Salisu 1Department of Pharmaceutical and Medicinal Chemistry, Faculty of Pharmaceutical Sciences,\u00a0Usmanu Danfodiyo University, Sokoto, Nigeria. \u00a0 2Department of Pharmaceutical and Medicinal Chemistry, Faculty of Pharmaceutical Sciences,\u00a0Ahmadu Bello University, Zaria, […]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"om_disable_all_campaigns":false,"_monsterinsights_skip_tracking":false,"_monsterinsights_sitenote_active":false,"_monsterinsights_sitenote_note":"","_monsterinsights_sitenote_category":0,"footnotes":""},"categories":[209],"tags":[],"aioseo_notices":[],"_links":{"self":[{"href":"https:\/\/wapcpharm.org\/journal\/wp-json\/wp\/v2\/posts\/3852"}],"collection":[{"href":"https:\/\/wapcpharm.org\/journal\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/wapcpharm.org\/journal\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/wapcpharm.org\/journal\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/wapcpharm.org\/journal\/wp-json\/wp\/v2\/comments?post=3852"}],"version-history":[{"count":4,"href":"https:\/\/wapcpharm.org\/journal\/wp-json\/wp\/v2\/posts\/3852\/revisions"}],"predecessor-version":[{"id":3858,"href":"https:\/\/wapcpharm.org\/journal\/wp-json\/wp\/v2\/posts\/3852\/revisions\/3858"}],"wp:attachment":[{"href":"https:\/\/wapcpharm.org\/journal\/wp-json\/wp\/v2\/media?parent=3852"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/wapcpharm.org\/journal\/wp-json\/wp\/v2\/categories?post=3852"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/wapcpharm.org\/journal\/wp-json\/wp\/v2\/tags?post=3852"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}