{"id":4360,"date":"2023-07-22T15:37:00","date_gmt":"2023-07-22T15:37:00","guid":{"rendered":"https:\/\/wapcpharm.org\/journal\/?p=4360"},"modified":"2023-07-27T13:47:00","modified_gmt":"2023-07-27T13:47:00","slug":"west-african-journal-of-pharmacy-2019-30-1-54-63-2","status":"publish","type":"post","link":"https:\/\/wapcpharm.org\/journal\/west-african-journal-of-pharmacy-2019-30-1-54-63-2\/","title":{"rendered":"West African Journal of Pharmacy (2019) 30 (1) 54 – 63"},"content":{"rendered":"\t\t
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Anti-inflammatory activity of the fruit extracts of Carpolobia lutea G. DON (Polygalaceae)<\/b><\/p>

Oghenekevwe M. Rhuada , Ogacheko D. Okoko , Gloria A. Ayoola , Ismaila O. Ishola<\/b><\/p>

Department of Pharmaceutical Chemistry, Faculty of Pharmacy, University of Lagos, PMB 12003, Lagos State,<\/span><\/p>

Nigeria. Department of Pharmacology, Therapeutics and Toxicology, Faculty of Basic Medical Sciences, College of<\/span><\/p>

Medicine, University of Lagos, PMB 12003, Lagos State, Nigeria.<\/p>

Corresponding author:<\/b> Gloria A. Ayoola<\/i><\/p>

Email:<\/b> gayoola@unilag.edu.ng<\/i>; Phone:<\/b> +2348063075179<\/i><\/p>\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t

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ABSTRACT<\/h2>\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t
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Background:<\/b> Carpolobia luteaG. Don (Polygalaceae) fruits are used traditionally in treating fever, infections and\u00a0inflammatory conditions.<\/p>

Objectives:<\/b> The study sought to evaluate the phytochemical constituents and anti-inflammatory potentials of\u00a0the fruit extracts of C. lutea.<\/p>

Methods:<\/b> Successive extraction was done with methanol (CLFME), n-hexane (CLFn-HE), chloroform (CLFCHE), and ethylacetate (CLFETE). Anti-inflammatory activity was evaluated in vivo using carrageenan-induced paw\u00a0oedema and xylene-induced ear oedema models. The phytochemical constituents were identified using gaschromatography (GC) and liquid chromatography – mass spectrometry (LC-MS).<\/p>

Results:<\/b> CLFME (200 mg\/kg) significantly (P< 0.05) inhibited carrageenan-induced paw oedema (83.66%) and\u00a0xylene induced ear oedema (89.85%). CLFn-HE, CLFCHE, and CLFETE (200 mg\/kg) significantly reduced\u00a0carrageenan-induced paw oedema with percentage inhibitions of 60.00, 70.00 and 65.00%, respectively.
Moreover, CLFn-HE, CLFCHE, and CLFETE (200 mg\/kg), reduced xylene-induced ear oedema by 69.14, 71.60,\u00a0and 69.14%, respectively. Gas chromatography coupled to mass spectroscopic (GC\u2013MS) analysis of CLFn-HE\u00a0afforded oleic acid, squalene, cis-vaccenic acid, n-hexadecanoic acid, 9-octadecenoic acid (Z) methyl ester, cis13-octadecenoic acid and nonacosane. CLFCHE was subjected to column chromatography, fraction-31 showed
a single spot using Thin layer chromatography (TLC) (n-hexane:ethylacetate, 5:5) with an R value of 0.70. Liquid chromatography coupled to mass spectroscopic (LC-MS) analysis of fraction-31 afforded four peaks with\u00a0retention times of 0.058, 1.161, 1.572 and 3.080 min, with similar abundant ions (Base peak) of 81.1 and\u00a0+ molecular ion (M ) of 208.2 indicating the presence of isomeric compounds.<\/p>

Conclusion:<\/b> The fruit extract of C. lutea possess anti-inflammatory effects which may be mediated through\u00a0inhibition or release of inflammatory mediators. Thus, C. lutea fruit extract could be a potential\u00a0phytotherapeutic agent in the management of inflammatory disorders.<\/p>

Keywords: <\/b>carrageenan; xylene; paw oedema; flavonoids; mass spectroscopy; chromatography<\/p>\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t

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\n\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\tDownload <\/span>\n\t\t<\/span>\n\t\t\t\t\t<\/a>\n\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t\t\t\t<\/div>\n\t\t","protected":false},"excerpt":{"rendered":"

Anti-inflammatory activity of the fruit extracts of Carpolobia lutea G. DON (Polygalaceae) Oghenekevwe M. Rhuada , Ogacheko D. Okoko , Gloria A. Ayoola , Ismaila O. Ishola Department of Pharmaceutical Chemistry, Faculty of Pharmacy, University of Lagos, PMB 12003, Lagos State, Nigeria. Department of Pharmacology, Therapeutics and Toxicology, Faculty of Basic Medical Sciences, College of […]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"om_disable_all_campaigns":false,"_monsterinsights_skip_tracking":false,"_monsterinsights_sitenote_active":false,"_monsterinsights_sitenote_note":"","_monsterinsights_sitenote_category":0,"footnotes":""},"categories":[211],"tags":[],"aioseo_notices":[],"_links":{"self":[{"href":"https:\/\/wapcpharm.org\/journal\/wp-json\/wp\/v2\/posts\/4360"}],"collection":[{"href":"https:\/\/wapcpharm.org\/journal\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/wapcpharm.org\/journal\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/wapcpharm.org\/journal\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/wapcpharm.org\/journal\/wp-json\/wp\/v2\/comments?post=4360"}],"version-history":[{"count":4,"href":"https:\/\/wapcpharm.org\/journal\/wp-json\/wp\/v2\/posts\/4360\/revisions"}],"predecessor-version":[{"id":4365,"href":"https:\/\/wapcpharm.org\/journal\/wp-json\/wp\/v2\/posts\/4360\/revisions\/4365"}],"wp:attachment":[{"href":"https:\/\/wapcpharm.org\/journal\/wp-json\/wp\/v2\/media?parent=4360"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/wapcpharm.org\/journal\/wp-json\/wp\/v2\/categories?post=4360"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/wapcpharm.org\/journal\/wp-json\/wp\/v2\/tags?post=4360"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}