{"id":4391,"date":"2023-07-18T16:15:28","date_gmt":"2023-07-18T16:15:28","guid":{"rendered":"https:\/\/wapcpharm.org\/journal\/?p=4391"},"modified":"2023-07-27T13:44:15","modified_gmt":"2023-07-27T13:44:15","slug":"west-african-journal-of-pharmacy-2019-30-1-104-118","status":"publish","type":"post","link":"https:\/\/wapcpharm.org\/journal\/west-african-journal-of-pharmacy-2019-30-1-104-118\/","title":{"rendered":"West African Journal of Pharmacy (2019) 30 (1) 104 – 118"},"content":{"rendered":"\t\t
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Chromosomal and histopathological alterations in Clarias gariepinus (Siluriformes, Clariidae) following<\/strong>
exposure to Shea butter effluent<\/strong><\/p>

Olufemi D. Owolabi , Oluyinka A. Iyiola<\/em>

Fisheries and Hydrobiology Research Laboratory, Department of Zoology, University of Ilorin, P.M.B 1515, Ilorin, Kwara State, Nigeria.

Cell Biology and Genetics Unit, Department of Zoology, University of Ilorin, P.M.B 1515, Ilorin, Kwara State,
Nigeria.<\/p>

Corresponding author:<\/strong> Olufemi D. Owolabi<\/em>
E-mail:<\/strong> olulabi@unilorin.edu.ng<\/em>; Phone:<\/strong> +2348034313927,<\/em><\/p>\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t

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ABSTRACT<\/h2>\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t
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Background:<\/b> Aquatic ecosystems have been a consistent recipient of industrial effluents capable of inducing\u00a0genotoxic and histopathological effects on aquatic organisms if untreated.<\/p>

Objectives: <\/b>The aim of this study was to examine the genotoxic, using chromosomal aberration (CA) assay, and\u00a0histopathological effects of Shea butter effluent (SBE) on Clarias gariepinus; one of the most widely consumed\u00a0fish in Nigeria.<\/p>

Methods:<\/b> Fish were exposed to different lethal concentrations (0.00, 0.045, 0.050, 0.055, 0.060 g\/l) for 96\u00a0hours and varying chronic concentrations (0.00, 0.0045, 0.0050, 0.0055, 0.0060 g\/l) of SBE for 14, 21 and 28\u00a0days after range finding test. Fish were intraperitoneally-injected with 0.05% colchicine 2-3 hours before\u00a0sacrifice. Physico-chemical parameters and heavy metal contents of the SBE were determined using standard\u00a0procedures. After exposure, fish were sacrificed and dissected to remove the gills, liver and kidney from both\u00a0the experimental and control groups. These organs were analysed for chromosomal aberrations and\u00a0histopathological lesions.<\/p>

Results:<\/b> There was a significant increase in CA frequency (P < 0.05) induced by SBE in gills, liver and kidney\u00a0among the treated groups compared with the control. Various types of CA observed were chromatids gaps and\u00a0deletions, acentric, dicentric and ring chromosomes. Histopathological analyses revealed epithelial lifting,
necrosis, lamellar fusion, oedema and haemorrhage in the gill, while vacuolation, hyperplasia, sinusoids,\u00a0hypertrophy and necrosis were observed in the liver.<\/p>

Conclusion:<\/b> This study suggests that untreated SBE contains potentially genotoxic and clastogenic substances
capable of inducing DNA damage and histopathological lesions in C. gariepinus.
Key words: Shea butter, effluent, chromosome aberration, histopathology, Clarias gariepinus<\/p>\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t

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\n\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\tDownload <\/span>\n\t\t<\/span>\n\t\t\t\t\t<\/a>\n\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t\t\t\t<\/div>\n\t\t","protected":false},"excerpt":{"rendered":"

Chromosomal and histopathological alterations in Clarias gariepinus (Siluriformes, Clariidae) followingexposure to Shea butter effluent Olufemi D. Owolabi , Oluyinka A. Iyiola Fisheries and Hydrobiology Research Laboratory, Department of Zoology, University of Ilorin, P.M.B 1515, Ilorin, Kwara State, Nigeria. Cell Biology and Genetics Unit, Department of Zoology, University of Ilorin, P.M.B 1515, Ilorin, Kwara State,Nigeria. Corresponding […]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"om_disable_all_campaigns":false,"_monsterinsights_skip_tracking":false,"_monsterinsights_sitenote_active":false,"_monsterinsights_sitenote_note":"","_monsterinsights_sitenote_category":0,"footnotes":""},"categories":[211],"tags":[],"aioseo_notices":[],"_links":{"self":[{"href":"https:\/\/wapcpharm.org\/journal\/wp-json\/wp\/v2\/posts\/4391"}],"collection":[{"href":"https:\/\/wapcpharm.org\/journal\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/wapcpharm.org\/journal\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/wapcpharm.org\/journal\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/wapcpharm.org\/journal\/wp-json\/wp\/v2\/comments?post=4391"}],"version-history":[{"count":4,"href":"https:\/\/wapcpharm.org\/journal\/wp-json\/wp\/v2\/posts\/4391\/revisions"}],"predecessor-version":[{"id":4396,"href":"https:\/\/wapcpharm.org\/journal\/wp-json\/wp\/v2\/posts\/4391\/revisions\/4396"}],"wp:attachment":[{"href":"https:\/\/wapcpharm.org\/journal\/wp-json\/wp\/v2\/media?parent=4391"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/wapcpharm.org\/journal\/wp-json\/wp\/v2\/categories?post=4391"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/wapcpharm.org\/journal\/wp-json\/wp\/v2\/tags?post=4391"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}