{"id":4727,"date":"2023-07-26T13:50:00","date_gmt":"2023-07-26T13:50:00","guid":{"rendered":"https:\/\/wapcpharm.org\/journal\/?p=4727"},"modified":"2023-07-31T13:54:44","modified_gmt":"2023-07-31T13:54:44","slug":"west-african-journal-of-pharmacy-2016-27-2-65-77","status":"publish","type":"post","link":"https:\/\/wapcpharm.org\/journal\/west-african-journal-of-pharmacy-2016-27-2-65-77\/","title":{"rendered":"West African Journal of Pharmacy (2016) 27 (2) 65-77"},"content":{"rendered":"\t\t
\n\t\t\t\t\t\t\t\t\t
\n\t\t\t\t\t\t
\n\t\t\t\t\t
\n\t\t\t
\n\t\t\t\t\t\t\t\t
\n\t\t\t\t
\n\t\t\t\t\t\t\t

Effects of the extracts and derived fractions of four medicinal plants on hepatic microsomal enzymes:\u00a0<\/b>an in-vitro study<\/b><\/p>

\u00a0<\/p>

Abidemi J. Akindele , Olufunmilayo O. Adeyemi and Margaret O. James<\/p>

\u00a0<\/p>

1Department of Pharmacology, Therapeutics & Toxicology (PTT), Faculty of Basic Medical Sciences, College of\u00a0<\/span>Medicine, University of Lagos, Idi-Araba Campus, P. M. B. 12003, Lagos, Nigeria<\/span><\/p>

\u00a0<\/p>

2Department of Medicinal Chemistry, College of Pharmacy, University of Florida, Gainesville, FL 32610-0485, USA<\/span><\/p>

\u00a0<\/p>

Corresponding author: Abidemi J. Akindele<\/p>

E-mail : jakindele@unilag.edu.ng Phone: +2348062359726<\/p>\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t

\n\t\t\t\t\t\t
\n\t\t\t\t\t
\n\t\t\t
\n\t\t\t\t\t\t\t\t
\n\t\t\t\t
\n\t\t\t

ABSTRACT<\/h2>\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t
\n\t\t\t\t\t\t
\n\t\t\t\t\t
\n\t\t\t
\n\t\t\t\t\t\t\t\t
\n\t\t\t\t
\n\t\t\t\t\t\t\t

Background:<\/b> The use of medicinal plants for treatment of diverse ailments has continued to increase worldwide, especially in developing countries. The concurrent use of herbal medicines and conventional drugs is common. <\/span>Herb-drug interaction through the inhibition\/induction of cytochrome (CYP) P450 metabolizing enzymes could <\/span>result in pharmacotoxicity\/therapeutic failure.<\/span><\/p>

Objective: <\/b>This study investigated the extracts and fractions of four medicinal plants (Crinum glaucum, Baphia nitida, Byrsocarpus coccineus, and Sanseviera liberica) for possible effects on hepatic microsomal enzymes in-<\/span>vitro.<\/span><\/p>

Methods:<\/b> The benzyloxy-4-[trifluoromethyl]-coumarin O-debenzyloxylase (BFCOD; CYP3A4) and <\/span>benzyloxyresorufin O-debenzylase (BROD; CYP2B1\/2) assays were used.<\/span><\/p>

Results:<\/b> The ethyl acetate:methanol and ethanol:water extracts of the plants demonstrated significant (p < 0.05) inhibitory effect on CYP activity, pronouncedly at the highest concentration (1 mg\/ml) in the BROD assay. The <\/span>aqueous extracts and fractions of all the plants did not significantly affect CYP activity in both assays. The same <\/span>trend of observation was recorded in the BFCOD assay except that the ethyl acetate:methanol extract of C. <\/span>glaucum did not significantly affect CYP activity. The ethyl acetate:methanol extract of B. nitida and B. coccineus <\/span>elicited significant stimulatory effect at 0.01 and 0.1 mg\/ml in the BROD assay. The hexane and n-butanol <\/span>fractions of the extracts showed varying tendency to cause stimulation of CYP activity at lower doses and <\/span>inhibition at the highest dose.<\/span><\/p>

Conclusion: <\/b>Findings suggest that the aqueous extracts\/fractions of the plants investigated have little or no tendency to significantly affect CYP activity. Other organic solvent extracts\/fractions possess the ability to inhibit <\/span>CYP activity, especially at high doses.<\/span><\/p>

Keywords:<\/b> Crinum glaucum, Baphia nitida, Byrsocarpus coccineus, Sanseviera liberica, Herb-drug interaction, Cytochrome P450 enzymes<\/span><\/p>\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t

\n\t\t\t\t
\n\t\t\t\t\t
\n\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\tDownload <\/span>\n\t\t<\/span>\n\t\t\t\t\t<\/a>\n\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t\t\t\t<\/div>\n\t\t","protected":false},"excerpt":{"rendered":"

Effects of the extracts and derived fractions of four medicinal plants on hepatic microsomal enzymes:\u00a0an in-vitro study \u00a0 Abidemi J. Akindele , Olufunmilayo O. Adeyemi and Margaret O. James \u00a0 1Department of Pharmacology, Therapeutics & Toxicology (PTT), Faculty of Basic Medical Sciences, College of\u00a0Medicine, University of Lagos, Idi-Araba Campus, P. M. B. 12003, Lagos, Nigeria […]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"om_disable_all_campaigns":false,"_monsterinsights_skip_tracking":false,"_monsterinsights_sitenote_active":false,"_monsterinsights_sitenote_note":"","_monsterinsights_sitenote_category":0,"footnotes":""},"categories":[225],"tags":[],"aioseo_notices":[],"_links":{"self":[{"href":"https:\/\/wapcpharm.org\/journal\/wp-json\/wp\/v2\/posts\/4727"}],"collection":[{"href":"https:\/\/wapcpharm.org\/journal\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/wapcpharm.org\/journal\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/wapcpharm.org\/journal\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/wapcpharm.org\/journal\/wp-json\/wp\/v2\/comments?post=4727"}],"version-history":[{"count":4,"href":"https:\/\/wapcpharm.org\/journal\/wp-json\/wp\/v2\/posts\/4727\/revisions"}],"predecessor-version":[{"id":4733,"href":"https:\/\/wapcpharm.org\/journal\/wp-json\/wp\/v2\/posts\/4727\/revisions\/4733"}],"wp:attachment":[{"href":"https:\/\/wapcpharm.org\/journal\/wp-json\/wp\/v2\/media?parent=4727"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/wapcpharm.org\/journal\/wp-json\/wp\/v2\/categories?post=4727"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/wapcpharm.org\/journal\/wp-json\/wp\/v2\/tags?post=4727"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}