{"id":4938,"date":"2023-07-27T15:16:48","date_gmt":"2023-07-27T15:16:48","guid":{"rendered":"https:\/\/wapcpharm.org\/journal\/?p=4938"},"modified":"2023-08-03T15:22:23","modified_gmt":"2023-08-03T15:22:23","slug":"west-african-journal-of-pharmacy-2015-26-2-83-91","status":"publish","type":"post","link":"https:\/\/wapcpharm.org\/journal\/west-african-journal-of-pharmacy-2015-26-2-83-91\/","title":{"rendered":"West African Journal of Pharmacy (2015) 26 (2) 83-91"},"content":{"rendered":"\t\t
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Free radical scavenging and cytotoxic effects of methanol extract of Theobroma cacao L. (Sterculiaceae) seed<\/b><\/p>

\u00a0<\/p>

Kelly Oriakhi , Osayemwenre Erharuyi , Ehigbai I. Oikeh , Nadja Engel-Lutz and Abiodun Falodun<\/p>

1Department of Medical Biochemistry, Faculty of Basic Medical Sciences, University of Benin, Nigeria<\/span><\/p>

2Department of Pharmaceutical Chemistry, Faculty of Pharmacy, University of Benin, Nigeria<\/span><\/p>

3Department of Biochemistry, Faculty of Life Sciences, University of Benin, Nigeria<\/span><\/p>

4Department of Cell Biology, Institute of Biomedical Research, University of Rostock, Germany<\/span><\/p>

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Corresponding author: Ehigbai Oikeh<\/p>

Email: ehigbai.oikeh@uniben.edu Phone: +234(0)8025627009<\/p>\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t

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ABSTRACT<\/h2>\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t
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Background: <\/b>Theobroma cacao L. (Sterculiaceae) is a plant with great economic and health benefits. The plant is believed to possess diverse medicinal value among different populations.<\/span><\/p>\n

Objective:<\/b> The present study determined the radical scavenging and cytotoxic activities of the methanol seed extract of Theobroma cacao and its fractions.<\/span><\/p>\n

Methods:<\/b> Total phenolic, flavonoid content and the 2,2-diphenyl-2-picryl-hydrazyl (DPPH) radical spectrophotometric assays were used to determine the antioxidant potential of the T. cacao seed extracts. <\/span>Cytotoxicity activity of the extracts was determined using the MTS assay against MCF-7 breast cancer cell <\/span>lines.<\/span><\/p>\n

Results:<\/b> The seed extracts showed remarkably high scavenging effect on DPPH radical. The ethyl acetate fraction exhibited the highest scavenging effect with IC value of 5.00 \u03bcg\/mL. The phenolic and flavonoid 50 <\/span>contents were significantly higher in the ethyl acetate fraction compared to other fractions (p < 0.05). The <\/span>MTS assay revealed about 17.90 % and 22.00 % reduction in MCF-7 viability after 48 h exposure to 50 \u03bcg\/ml of <\/span>the crude methanol extract and the petroleum ether fraction respectively.<\/span><\/p>\n

Conclusion:<\/b> The above results illustrate the antioxidant ability of cocoa seed and show its potential cytotoxic effect on breast cancer cell line. This finding however needs substantiation by further experiments.<\/span><\/p>\n

Key words:<\/b> Theobroma cacao,radical scavenging, DPPH, cytotoxicity<\/p>\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t

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Free radical scavenging and cytotoxic effects of methanol extract of Theobroma cacao L. (Sterculiaceae) seed \u00a0 Kelly Oriakhi , Osayemwenre Erharuyi , Ehigbai I. Oikeh , Nadja Engel-Lutz and Abiodun Falodun 1Department of Medical Biochemistry, Faculty of Basic Medical Sciences, University of Benin, Nigeria 2Department of Pharmaceutical Chemistry, Faculty of Pharmacy, University of Benin, Nigeria […]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"om_disable_all_campaigns":false,"_monsterinsights_skip_tracking":false,"_monsterinsights_sitenote_active":false,"_monsterinsights_sitenote_note":"","_monsterinsights_sitenote_category":0,"footnotes":""},"categories":[224],"tags":[],"aioseo_notices":[],"_links":{"self":[{"href":"https:\/\/wapcpharm.org\/journal\/wp-json\/wp\/v2\/posts\/4938"}],"collection":[{"href":"https:\/\/wapcpharm.org\/journal\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/wapcpharm.org\/journal\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/wapcpharm.org\/journal\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/wapcpharm.org\/journal\/wp-json\/wp\/v2\/comments?post=4938"}],"version-history":[{"count":4,"href":"https:\/\/wapcpharm.org\/journal\/wp-json\/wp\/v2\/posts\/4938\/revisions"}],"predecessor-version":[{"id":4944,"href":"https:\/\/wapcpharm.org\/journal\/wp-json\/wp\/v2\/posts\/4938\/revisions\/4944"}],"wp:attachment":[{"href":"https:\/\/wapcpharm.org\/journal\/wp-json\/wp\/v2\/media?parent=4938"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/wapcpharm.org\/journal\/wp-json\/wp\/v2\/categories?post=4938"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/wapcpharm.org\/journal\/wp-json\/wp\/v2\/tags?post=4938"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}