{"id":5004,"date":"2023-07-30T15:48:14","date_gmt":"2023-07-30T15:48:14","guid":{"rendered":"https:\/\/wapcpharm.org\/journal\/?p=5004"},"modified":"2023-08-04T16:10:19","modified_gmt":"2023-08-04T16:10:19","slug":"west-african-journal-of-pharmacy-2015-26-1-41-53","status":"publish","type":"post","link":"https:\/\/wapcpharm.org\/journal\/west-african-journal-of-pharmacy-2015-26-1-41-53\/","title":{"rendered":"West African Journal of Pharmacy (2015) 26 (1) 41-53"},"content":{"rendered":"\t\t
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Simultaneous quantification of five penicillins and detection of penicilloic acid in single\u00a0<\/b>and\/or multi\u2013 component flucloxacillin pharmaceutical formulations<\/b><\/p>

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Mahmood B. Oppong , Samuel O. Bekoe , Reimel K. Adosraku , John S.K. Ayim<\/p>

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1Department of Pharmaceutical Chemistry, School of Pharmacy, University of Ghana, Legon, Accra<\/span><\/p>

2Department of Pharmaceutical Chemistry, Faculty of Pharmacy and Pharmaceutical Sciences,\u00a0<\/span>Kwame Nkrumah University of Science and Technology (KNUST), Kumasi, Ghana<\/span><\/p>

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Corresponding author: Mahmood Oppong<\/p>

Email: mahmood.obrobbey@gmail.com Phone: +233(0)208360510<\/p>\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t

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ABSTRACT<\/h2>\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t
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Background:<\/b> The role of \u03b2-lactams among antibacterial agents in the treatment and management of\u00a0infectious diseases cannot be under-estimated. Swift actions regarding quality assessment of penicillins (most\u00a0<\/span>frequently prescribed \u03b2-lactam) supplied to patients especially in circumstances where the products do not\u00a0<\/span>conform to specifications remain a challenge, i.e. inability to detect quality products from\u00a0<\/span>counterfeit\/substandard ones.<\/span><\/p>

Objectives<\/b>: The goals of this study were to develop a simple RP-HPLC method for the simultaneous\u00a0quantification of amoxicillin trihydrate (AXT), ampicillin trihydrate (APT), benzylpenicillin sodium (BPS),\u00a0<\/span>flucloxacillin sodium (FXS) and phenoxymethyl penicillin sodium (PMS) in single and combination dosage\u00a0<\/span>forms, detect substandard penicillin antibacterial agents and also detect penicilloic acid, a major breakdown\u00a0<\/span>product of penicillins and a major semi-synthetic precursor of penicillins.<\/span><\/p>

Methods: Separation was achieved on a Phenomenex LICHROSORB 10 RP-1 C column (250 \u00d7 4.60 mm I.D., 5 18\u00a0\u03bcm particle size) with methanol: 0.02M KH PO (pH = 3.70) (4:6v\/v) in isocratic mode as mobile phase. A flow 2 4\u00a0<\/span>rate of 1.00 mL\/min with UV detection at 225 nm was optimized, validated, and employed to assay samples\u00a0<\/span>from the Ghanaian market.<\/span><\/p>

Results:<\/b> The calibration plots for the determination of the penicillins showed correlation values (r ) between\u00a00.9984 – 0.9995 in the concentration range of 0.005 – 0.1%w\/v. Accuracy for the analytical method was\u00a0<\/span>confirmed with mean recoveries (n=3) in the range of 99.88 – 101.33%, 100.01 – 100.66% and 99.10 – 100.79%\u00a0<\/span>at concentration levels of 80, 100 and 120% respectively. Method precision with relative standard deviation\u00a0<\/span>(RSD) obtained for intra-day precision were 1.62 – 4.03% and the inter-day precision were 1.22 \u2013 3.97%.\u00a0<\/span>The developed method was found suitable for the detection of penicilloic acid and could distinguish between\u00a0<\/span>good quality and substandard penicillin antibacterial products sampled from Kumasi metropolis.<\/span><\/p>

Conclusion:<\/b> The method is suitable for the simultaneous assay of AXT, APT, BPS, PMS and FXS in either\u00a0single\/multi \u2013 component dosage forms. It is also applicable for routine screening of penicillin antibiotics for\u00a0<\/span>adulteration and counterfeiting and the detection of penicilloic acid, a major decomposition product in\u00a0<\/span>penicillins.<\/span><\/p>

Key words:<\/b> Penicillins, Impurity profiling, RP-HPLC, Isocratic mode, Validation<\/p>\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t

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\n\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\tDownload <\/span>\n\t\t<\/span>\n\t\t\t\t\t<\/a>\n\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t\t\t\t<\/div>\n\t\t","protected":false},"excerpt":{"rendered":"

Simultaneous quantification of five penicillins and detection of penicilloic acid in single\u00a0and\/or multi\u2013 component flucloxacillin pharmaceutical formulations \u00a0 Mahmood B. Oppong , Samuel O. Bekoe , Reimel K. Adosraku , John S.K. Ayim \u00a0 1Department of Pharmaceutical Chemistry, School of Pharmacy, University of Ghana, Legon, Accra 2Department of Pharmaceutical Chemistry, Faculty of Pharmacy and Pharmaceutical […]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"om_disable_all_campaigns":false,"_monsterinsights_skip_tracking":false,"footnotes":""},"categories":[234],"tags":[],"aioseo_notices":[],"_links":{"self":[{"href":"https:\/\/wapcpharm.org\/journal\/wp-json\/wp\/v2\/posts\/5004"}],"collection":[{"href":"https:\/\/wapcpharm.org\/journal\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/wapcpharm.org\/journal\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/wapcpharm.org\/journal\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/wapcpharm.org\/journal\/wp-json\/wp\/v2\/comments?post=5004"}],"version-history":[{"count":4,"href":"https:\/\/wapcpharm.org\/journal\/wp-json\/wp\/v2\/posts\/5004\/revisions"}],"predecessor-version":[{"id":5010,"href":"https:\/\/wapcpharm.org\/journal\/wp-json\/wp\/v2\/posts\/5004\/revisions\/5010"}],"wp:attachment":[{"href":"https:\/\/wapcpharm.org\/journal\/wp-json\/wp\/v2\/media?parent=5004"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/wapcpharm.org\/journal\/wp-json\/wp\/v2\/categories?post=5004"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/wapcpharm.org\/journal\/wp-json\/wp\/v2\/tags?post=5004"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}