{"id":5098,"date":"2023-07-22T15:35:15","date_gmt":"2023-07-22T15:35:15","guid":{"rendered":"https:\/\/wapcpharm.org\/journal\/?p=5098"},"modified":"2023-08-05T15:43:35","modified_gmt":"2023-08-05T15:43:35","slug":"west-african-journal-of-pharmacy-2022-33-2-76-89-2","status":"publish","type":"post","link":"https:\/\/wapcpharm.org\/journal\/west-african-journal-of-pharmacy-2022-33-2-76-89-2\/","title":{"rendered":"West African Journal of Pharmacy (2022) 33 (2) 76 – 89"},"content":{"rendered":"\t\t
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In vitro and in vivo antifungal activities of Boerhavia diffusa Linn on selected dermatophyte strains<\/b><\/p>\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t

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ABSTRACT<\/h2>\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t
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Background:<\/b> Dermatophytic infections have been considered to be a major public health problem. The\u00a0emergence of recalcitrant dermatophytosis, coupled with resistance of dermatophytes to existing antifungal\u00a0<\/span>agents has necessitated the search for efficacious agents. Boerhavia diffusa is used as an ethnomedicinal plant.<\/span><\/p>

Objective:<\/b> To investigate the antidermatophytic activity of the extracts and ointment formulation of Boerhavia\u00a0diffusa<\/span><\/p>

Methods<\/b>: Boerhavia diffusa leaves were extracted with hexane, ethyl acetate and methanol using a soxhlet\u00a0apparatus. Fractionation of extracts was done using Vacuum Liquid Chromatography. Twelve dermatophyte\u00a0<\/span>isolates were used for the study. Agar well diffusion and broth microbroth dilution methods were used for the\u00a0<\/span>antifungal assay of extracts and fractions and to determine the minimum inhibitory and minimum fungicidal\u00a0<\/span>concentrations. Crude leaf extract was formulated into water based ointment at concentrations of 0.5, 1 and 1.5\u00a0<\/span>mg\/ml. Antidermatophytic activity of the ointment formulation was carried out using Wistar rats infected with\u00a0<\/span>Trychophyton rubrum ATCC 28188 and Epidermophyton flocossum. Haematological and histopathological\u00a0<\/span>evaluations of animals were carried out.<\/span><\/p>

Results<\/b>: MIC and MFC of extracts ranged between 1.56 mg\/ml – 50 mg\/ml and 25 mg\/ml – 100 mg\/ml respectively.\u00a0The fractions showed highest inhibitory activities against Microsporum audouinii. Total clearance of infection\u00a0<\/span>with ointment formulation (1.5 mg\/ml) was achieved in one of the infected groups while clearance by\u00a0<\/span>ketoconazole was achieved in all infected groups. Haematological studies revealed a significant increase in white\u00a0<\/span>blood cells of the infected animals after treatment. Histopathological studies showed moderate epidermal\u00a0<\/span>hyperplasia.<\/span><\/p>

Conclusion: <\/b>This study revealed that Boerhavia diffusa has antidermatophytic activities.<\/p>

Keywords:<\/b> Boerhavia diffusa,Dermatophytes, Ointment, Haematological, Histopathological<\/p>\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t

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\n\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\tDownload <\/span>\n\t\t<\/span>\n\t\t\t\t\t<\/a>\n\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t\t\t\t<\/div>\n\t\t","protected":false},"excerpt":{"rendered":"

In vitro and in vivo antifungal activities of Boerhavia diffusa Linn on selected dermatophyte strains ABSTRACT Background: Dermatophytic infections have been considered to be a major public health problem. The\u00a0emergence of recalcitrant dermatophytosis, coupled with resistance of dermatophytes to existing antifungal\u00a0agents has necessitated the search for efficacious agents. Boerhavia diffusa is used as an ethnomedicinal […]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"om_disable_all_campaigns":false,"_monsterinsights_skip_tracking":false,"_monsterinsights_sitenote_active":false,"_monsterinsights_sitenote_note":"","_monsterinsights_sitenote_category":0,"footnotes":""},"categories":[1],"tags":[],"aioseo_notices":[],"_links":{"self":[{"href":"https:\/\/wapcpharm.org\/journal\/wp-json\/wp\/v2\/posts\/5098"}],"collection":[{"href":"https:\/\/wapcpharm.org\/journal\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/wapcpharm.org\/journal\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/wapcpharm.org\/journal\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/wapcpharm.org\/journal\/wp-json\/wp\/v2\/comments?post=5098"}],"version-history":[{"count":4,"href":"https:\/\/wapcpharm.org\/journal\/wp-json\/wp\/v2\/posts\/5098\/revisions"}],"predecessor-version":[{"id":5104,"href":"https:\/\/wapcpharm.org\/journal\/wp-json\/wp\/v2\/posts\/5098\/revisions\/5104"}],"wp:attachment":[{"href":"https:\/\/wapcpharm.org\/journal\/wp-json\/wp\/v2\/media?parent=5098"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/wapcpharm.org\/journal\/wp-json\/wp\/v2\/categories?post=5098"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/wapcpharm.org\/journal\/wp-json\/wp\/v2\/tags?post=5098"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}