{"id":5264,"date":"2023-08-02T16:21:55","date_gmt":"2023-08-02T16:21:55","guid":{"rendered":"https:\/\/wapcpharm.org\/journal\/?p=5264"},"modified":"2023-08-07T16:26:04","modified_gmt":"2023-08-07T16:26:04","slug":"west-african-journal-of-pharmacy-2012-23-2-34-39","status":"publish","type":"post","link":"https:\/\/wapcpharm.org\/journal\/west-african-journal-of-pharmacy-2012-23-2-34-39\/","title":{"rendered":"West African Journal of Pharmacy (2012) 23 (2) 34 \u2013 39"},"content":{"rendered":"\t\t
\n\t\t\t\t\t\t\t\t\t
\n\t\t\t\t\t\t
\n\t\t\t\t\t
\n\t\t\t
\n\t\t\t\t\t\t\t\t
\n\t\t\t\t
\n\t\t\t\t\t\t\t

Validation of UV spectrophotometric method of analysis of some marketed perfloxacin in Nigeria<\/b><\/p>

\u00a0<\/b><\/p>

Obiageri .O. Obodozie , Florence Tarfa , Enoche Oga , Ozadheoghene .E. Afieroho *, and Christian .O. Onuh<\/p>

\u00a0<\/p>

1\u00a0Department of Medicinal Chemistry and Quality Control, National Institute for\u00a0<\/span>Pharmaceutical Research and Development, Idu, Abuja, Nigeria.<\/span><\/p>

2\u00a0Faculty of Pharmacy, University of Port-Harcourt, Nigeria.<\/span><\/p>

\u00a0<\/span><\/p>

*Corresponding Author: Ozadheoghene E. Afieroho<\/p>

E-mail: eriarieafieroho@yahoo.com; Tel: +2348063432417<\/p>\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t

\n\t\t\t\t\t\t
\n\t\t\t\t\t
\n\t\t\t
\n\t\t\t\t\t\t\t\t
\n\t\t\t\t
\n\t\t\t

ABSTRACT<\/h2>\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t
\n\t\t\t\t\t\t
\n\t\t\t\t\t
\n\t\t\t
\n\t\t\t\t\t\t\t\t
\n\t\t\t\t
\n\t\t\t\t\t\t\t

Background:<\/b> Acquiring sophisticated LC instruments by most third world laboratories is capital intensive. Literatures on simple spectrophotometric analytical methods for pefloxacin are scarce.<\/span><\/p>

Objectives:<\/b> The present study was undertaken to develop and validate a simple and economic UV spectrophotometric method for estimating pefloxacin mesylate (PFM) in dosage preparations<\/span><\/p>

Methods:<\/b> Using a JENWAY spectrophotometer at predetermined \u00ebmax of 277nm with 1% v\/v aqueous glacial acetic acid as blank, the method was validated for linearity, accuracy, precision, reproducibility, and specificity as <\/span>per International Conference on Harmonization (ICH) guidelines and used to determine the content of pefloxacin <\/span>in seven marketed brands in Nigeria.<\/span><\/p>

Results<\/b>: The method exhibited good linearity over a range of 0.40\u201312.0 \u03bcg\/ml (regression equation: y = 0.0859x+ 0.0211 ; r=0.999). Mean recovery accuracy (99.183%) and assay result in the range of 100.5- 110.17% for the <\/span>selected brands were not significantly different at p=0.05. The % coefficient of variation (CV) for both intra and <\/span>inter-day were below 7 %. The method was specific for pefloxacin in the presence of common excipients<\/span><\/p>

Conclusion: <\/b>The method gave good validation results and could be employed for routine analysis of PFM in commercial formulations.<\/span><\/p>

Key words<\/b>: Pefloxacin mesylate, spectrophotometric method, validation, and assay<\/p>\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t

\n\t\t\t\t
\n\t\t\t\t\t
\n\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\tDownload <\/span>\n\t\t<\/span>\n\t\t\t\t\t<\/a>\n\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t\t\t\t<\/div>\n\t\t","protected":false},"excerpt":{"rendered":"

Validation of UV spectrophotometric method of analysis of some marketed perfloxacin in Nigeria   Obiageri .O. Obodozie , Florence Tarfa , Enoche Oga , Ozadheoghene .E. Afieroho *, and Christian .O. Onuh 1 Department of Medicinal Chemistry and Quality Control, National Institute for Pharmaceutical Research and Development, Idu, Abuja, Nigeria. 2 Faculty of Pharmacy, University of Port-Harcourt, Nigeria. […]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"om_disable_all_campaigns":false,"_monsterinsights_skip_tracking":false,"_monsterinsights_sitenote_active":false,"_monsterinsights_sitenote_note":"","_monsterinsights_sitenote_category":0,"footnotes":""},"categories":[237],"tags":[],"aioseo_notices":[],"_links":{"self":[{"href":"https:\/\/wapcpharm.org\/journal\/wp-json\/wp\/v2\/posts\/5264"}],"collection":[{"href":"https:\/\/wapcpharm.org\/journal\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/wapcpharm.org\/journal\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/wapcpharm.org\/journal\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/wapcpharm.org\/journal\/wp-json\/wp\/v2\/comments?post=5264"}],"version-history":[{"count":5,"href":"https:\/\/wapcpharm.org\/journal\/wp-json\/wp\/v2\/posts\/5264\/revisions"}],"predecessor-version":[{"id":5271,"href":"https:\/\/wapcpharm.org\/journal\/wp-json\/wp\/v2\/posts\/5264\/revisions\/5271"}],"wp:attachment":[{"href":"https:\/\/wapcpharm.org\/journal\/wp-json\/wp\/v2\/media?parent=5264"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/wapcpharm.org\/journal\/wp-json\/wp\/v2\/categories?post=5264"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/wapcpharm.org\/journal\/wp-json\/wp\/v2\/tags?post=5264"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}